Isolation, molecular characterization and phylogenetic analysis of potential phosphate solubility bacteria from Oryza sativa

Abstract

Uptake and translocation of cationic and anionic nutrients play essential roles in physiological processes including plant growth, nutrition, signal transduction, and development. Approximately 5% of the Oryza sativa genome appears to encode membrane transport proteins. These proteins are classified and several hundred putative transporters have not yet been assigned to families. In this work, we have analyzed the phylogenetic relationships of cation/proton antiporter proteins, CNGC (cyclic nucleotide gated channel) and chloride channels which are anion transporters. This analysis has focused on cation and anion transporter gene families for which initial characterizations have been achieved for individual members, including sodium transporters, calcium antiporters, cyclic nucleotide-gated channels and chloride channels. Phylogenetic tree of each family define the evolutionary relationships of the members to each other. These families contain numerous members, indicating diverse functions in vivo. Closely related isoforms and separate subfamilies exist within many of these gene families, indicating possible redundancies and specialized functions. To facilitate their

further study, which include alignment of the analyzed genes and their chromosomal location cation,