Isolation and Identification of Microalgae from Fresh water Sources

Abstract

Isolation and characterization of microalgae was carried from pond and freshwater samples from Michael Okpara University of Agriculture umudike and Amafor isingwu  Umuahia respectively ., all located in Abia state , The samples were collected bimonthly for three months. Samples were cultured using media prepared from a fresh water base supplemented with growth factors essential for algal growth for incubation at 25-30 ̊ C for about 7-14days in the presence of light source.Algae were isolated from the sampled ponds but samples from the freshwater stream (Amafor stream) did not yield algal colonial colonies. Four different algal species were isolated from sites (Pond A&B), both sites showed the presence of Rhizosolenia longiseta zach and Cyclotella bodancia  eulenst. Site A had the highest number of algal species (four), while site B had only three different algal types. However, the physiochemical analysis of the pond and the stream values were gotten. The study concluded the presence of microalgae in ponds.