Plant regeneration through callus induction from nodal segments of Polygonum hydropiper L., an important medicinal plant

Abstract

The investigation was carried out to observed callus induction and subsequent regeneration potentiality of hydropepper (Polygonum hydropiper) from nodal segments of mature plants. Greenish friable callus was achieved from nodal segment on MS medium supplemented with 5.0mgl-12,4-D within two weeks  of inoculation. The callus produced large number of shoots when cultured on MS medium with 5.0 mgl-12,4- D +0.2 mgl-1Kn within 10 days of culture. In vitro raised shoots were rooted on MS medium with 1.0 mgl-1IBA within 15 days of culture. In vitro grown plantlets with well root system were successfully established in natural condition through successive phages of acclimatization. The survival rate of plantlets was found to be 100% in the natural condition.