Comparative Studies on the Total Phenolic and Antioxidant Activities of White and Red Pomelo Fruits

Abstract

In this study, the total phenolic content and antioxidant activities of two kinds of Citrus decumana Linn. (Pomelo) were comparatively determined by spectrophotometric methods. Total phenolic content of white and red fruit juice were determined by Folin-Ciocalteu reagent and UV spectrophotometer at 765 nm. The phenolic contents were calculated from the standard quarcetin curve which obtained the 1.58 mg/mL and 1.31 mg/mL. Moreover the antioxidant activities of these fruits were measured by DPPH radical scavenging assay method. IC₅₀ values of white and red pomelo fruits are 1.42 µg/mL and 2.75 µg/mL. According to the comparison of IC₅₀ values from experiment, white pomelo fruit has higher antioxidant activity than red pomelo fruit which indicates that the phenolic content of white pomelo fruit is slightly higher than that of red pomelo fruit.