Eco Freinfly Synthesis And Characterization Of Silver Nanoparticles Using Aquous Leaf Extract Of Alphonsea Sclerocarpa And Apply It As Antimicrobial

Abstract

To develop a reliable, eco-friendly and easy process for the synthesis of silver nanoparticles using leave extracts of medicinal plant “Alphonsea sclerocarpa”. There has been an exponentially increasing interest in biological synthesis of AgNPs. In this study, AgNPs were synthesized by an eco-friendly and convenient method using Alphonsea sclerocarpa leaf extract at ambient temperature. Alphonsea sclerocarpa leaf extract has been used as a reducing agent for the synthesis of silver nitrate into silver nanoparticles. Green synthesized silver nanoparticles are confirmed by color change which was monitored quantitatively by UV-Vis spectroscopy at 440 nm (Fig.4). Further characterization with SEM, TEM and AFM analysis shows the spherical, polydisperse AgNPs of particle size ranging from 5 to 50 nm with an average size of 28.5 nm (Fig.7,8,9). FTIR showed the structure, the respective bands of the synthesized nanoparticles, and the stretch of bonds. The cytotoxicity analysis of the green synthesized silver nanoparticles was observed that showed highest antimicrobial activity against gram positive bacteria Staphylococcus aureus (MTCC-6908) (Fig.11) showed average zone of inhibition of (26 mm), the Gram negative bacteria Pseudomonas citronellolis (MTCC-1191) (Fig.12) showed average zone of inhibition of (18.3 mm) and the Fungi Candida albicans (MTCC-4748) (Fig.13) showed average zone of inhibition of (22.2 mm) (Fig.10). However, further investigations were needed to identify the scaling-up usage of this extract on metallic nanoparticle synthesis and its applications as antimicrobial.