Isolation and Molecular Identification of Pathogenic Microbes From Hospital Burn Wounds by Polymerase Chain Reaction( Pcr )Technology

Abstract

Molecular Diagnostic Techniques are expected to play a significant role in clinical and diagnostic bacteriology. Although their adoption may never replace the conventional methods their efficiency, quality, quickness and their role in the detection of slow growing organisms cannot be overlooked. Infection control programs need to document and report burn wound infections according to the recently established definitions of the classification system. Future studies of burn wound infections should use this standardized burn wound classification system so that clinical outcomes can be compared for burn patients with a specific condition (e.g., burn wound cellulitis). More research is required to determine the best methods for sampling excised and unexcised burn wound areas over the course of a severe deep partial-thickness and/or full-thickness injury. Reproducible standardized methods should be developed so that clinical microbiology laboratories can routinely test burn wound bacterial isolates for susceptibility to the topical antimicrobial agents on formulary at a particular burn center. A rotation program for topical antimicrobial use may also retard the development of resistance. Laboratory surveillance should include the reporting of burn unit-specific antibiograms for topical antimicrobial agents once standardized methods are available for performing susceptibility testing.