DNA Binding Studies of Ni(Ii) Complex of (E-2-Chloro-N-(3-Hydroxy-5-Hydroxymethyl)-2-Methylpyridin-4-Yl)Methylene) Acetohydrazide

Abstract

(E-2-chloro-N-(3-hydroxy-5-hydroxymethyl)-2-methylpyridin-4yl)methylene) acetohydrazide, a derivative of pyridoxal was synthesized by conventional method by treating Pyridoxal hydrochloride with Chloroacetic hydrazide. Ni(II) metal complex was synthesized and characterized using LC-MS, UV, IR and TGA methods. IR spectrum of the complex revealed that the ligand coordinated through ‘N’ and ‘O’ donor atoms.

The complex was tested for DNA binding activity using Electronic Absorption spectroscopy and Viscosity measurements. The interaction of metal complexes with CT-DNA was studied and the binding constant (Kb) was calculated.