Bacterial Identification From Hydrocarbon Polluted Sources And Amplification Of Their Hydrocarbon Degrading Genes

Abstract

The present work deals with identification of bacteria present in different polluted sources followed by the amplification of their genes responsible for hydrocarbon degradation.Hydrocarbon plays a major role in environmental pollution leading to global warming, ozone depletion etc. Two different sources of hydrocarbon polluted samples viz Soil contaminated with Benzene and Water with Kerosine were undertaken for the identification of bacteria present in them. Following Bergey's manual of determinative bacteriology different biochemical tests were performed for bacterial identification along with gram staining and four different species of bacteria were identified as Micrococcus luteus, Streptococcus pyogenes, Bacillus subtilis and Corynebacterium kutsceri.The extent of degradation after 30 days was also estimated by PetroSensePHA-100WL.Using the literature search, respective genes responsible for hydrocarbon degradation were identified and amplified in these bacteria.