Molecular Profiling Of Rhizoshere Bacterial Communities of Plants Grown In Soils with Petroleum Contaminants

Abstract

Rhizosphere, a thin layer of soil that surrounds the root and is under the joint, direct influence of the root, root hairs, and AM hyphae adjacent to the root. In the rhizosphere, microbial processes include exudation, water uptake, nutrient mobilization, soil organic matter (SOM) decomposition, and respiration. The diversity of microbes associated with plant roots is enormous, in the order of tens of thousands of species. This complex plant-associated microbial community, also referred to as the second genome of the plant, is crucial for plant health. Recent advances in plant–microbe interactions research revealed that plants are able to shape their rhizosphere microbiome, as evidenced by the fact that different plant species host specific microbial communities when grown on the same soil. Understanding the rhizosphere interactions between microbes and plants is a critical first step in moving toward sustainable production of biofuel crops. In this project soil samples are procured from rhizoshere soil of soils having various petroleum contaminants. These soils are screened for isolation of bacteria and further studies are performed. DNA is isolated from the soil collected. The DNA is then amplified with the help of selected primers and agarose gel electrophoresis is run and then the target DNA is extracted from the gel.  Biochemical analysis of the bacteria is done to know the biochemical properties of the bacterial colonies from the soil. Further analysis is to be done.