Method Development and Validation for the Analysis of Perindopril Erbumine and Amlodipine Besilate by Rp-Hplc in Pure and Pharmaceutical Dosage Form

Abstract

The objective of this present work was to develop and validate analytical method for quantitative determination of Perindopril Erbumine and Amlodipine Besylate in a tablet formulation. Chromatographic separation of the two drugs was achieved on an Eclipse XDB C-8 (150 mm X 4.6 mm), 5mm. The mobile phase constituted of Buffer: Acetonitrile (65:35) and pH adjusted to 2.6 with dilute Ortho- Phosphoric Acid was delivered at the flow rate 1.0 mL/min. Detection was performed at 210 nm. Separation was completed within 8 min. Calibration curves were linear with correlation coefficient between 0.99 to 1.0 over a concentration range of 8 to 60 mg/mL of Perindopril Erbumine and 10 to 75 mg/mL of Amlodipine Besylate. The relative standard deviation (R.S.D) was found<2.0%.