P53 Alteration Studies and Tumor Suppressor Gene in Human Bladder Cancer of Subjects Exposed to High Levels of Chlordane

Abstract

The main objective of this work is to study the alterations that can occur in p53 in subjects exposed to chlordane. To study this various chlordane exposed subjects blood samples as well as bladder tissues are procured. Total blood protein is extracted from collected blood as well as from media from cultured cells. The main agenda behind such study is to identify for any mal formations in the p53 gene products in the extracted samples which do not have specificity to Anti p53 Antibody which in turn produces negative results in the immunological assays on the other hand those subjects which do not have any structural deformations in p53 protein produce a positive result in immunological assays using the same antibody. PCR is performed to the whole genomic DNA isolated from the subjects for amplification of p53 coding gene for cross referring the presence of the same in the amplified products. Various mixed results are procured out of which certain subjects have shown specific results which can conclude that p53 protein present in these subjects have nearly zero specificity to the Anti p53 Antibody which is used in the immunological assays for analyzing the same.