Quantitative Analysis Of The Expression Of P53 Gene In Colorectal Carcinoma By Using Real – Time PCR

Abstract

The established role of P53 is in Base Excision Repair of adenine residues that have been misincorporated opposite guanine or 8-oxoG (7, 8-dihydro-8-oxoguanine). The BER pathway plays a significant role in the repair of mutations caused by reactive oxygen species that are generated during aerobic metabolism Mutations in this gene affect the ability of cells to correct mistakes made during DNA replication biallelic mutations have been shown to predispose to an attenuated form of familial adenomatous polyposis (FAP)2 called P53. Most CRCs (colorectal cancers) are thought to develop from pre-existing adenomas, and CRC risk is increased in patients who have multiple adenomatous polyps in the colorectum. In the present work, the role of P53 gene in colon cancer has been evaluated. For this colon cancer HT 29 Colon cancer cells and MRC-5 fibroblast cells is collected. First the total RNA extraction is done, this total RNA is converted to cDNA to further carry out Real Time PCR analysis of the gene expression. As a result of the present study, it was observed that the gene expression levels of P53 are considerably decreased in colon cancer cell lines as compared to the normal cell lines. This result supports the previously carried out work on the role of P53 gene in colon cancer and predisposition to cancer in case of mutations in P53 gene.