Co-Relation of Biofilm Production with Multidrug Resistant Uropathogenic Klebsiella Pneumoniae Isolates and Their Antibiotic Susceptibility Patterns.

Abstract

BACKGROUND: Urinary tract infection because of antibiotic resistance is posing a serious health threat and biofilm formation being the leading root for the antibiotic resistance. The current study was intended to detect biofilm production by uropathogenic MDR klebsiella pneumoniae strains.

METHOD: The present study was conducted in the department of Microbiology of Maharishi Markandeshwar institute of medical science and research, Mullana, intended to detect biofilm formation in Multidrug-resistant uropathogenic Klebsiella pneumoniae isolates by using Tissue Culture plate Method, Tube adherence method and Modified Congo Red Agar Method.

RESULT: Out of total 550 samples, 23.2%(128) was uropathogenic. Out of 128 samples 60 samples were klebsiella pneumoniae strains, which were multidrug resistant (cephalosporins and floroquinolones). The rate of biofilm producers were 38(63.33%), detected by three methods. In which, Modified Congo Red Agar Method was best 36(60%) followed by Tube adherence method 33(55%) and Tissue Culture Plate Method 28(46.67%). In current study,the data on antibiogram revealed that Imipenem (73.68%) followed by Amikacin (68.42%) and Nitrofurantoin (52.63%) were most effective antibiotic for biofilm producing Multidrug-resistance Klebsiella pneumoniae.

CONCLUSION: So eventually we can conclude that the urine isolates especially Multidrug-resistance Klebsiella pneumoniae should be screened for biofilm production and antibiotic sensitivity testing should be done to determine antibiotic policy in the hospital.