Effect Of Hormone Concentrations And Age Of Explant On Multiple Shoot Regeneration From Cotyledon Of Cucumber

Abstract

The surface sterilized seeds of cucumber cv. Poinsett 76 were germinated on MS (Murashige and Skoog, 1962) basal medium supplemented with 3% sucrose and solidified with 0.7% (w/v) agar at 26± 2º C in light. Cotyledon  explants  excised from 5-day- old in vitro raised seedlings of cucumber (cucumissativus L.) were cultured on MS medium containing different concentrations of BAP(0-10 µM) .