Recombinant Cystatin-Like Thiol Proteinase Inhibitor (rCTPI) of Cicer arietinum (Chickpea): A potential source of Bio-pesticide.

Abstract

Cystatins are thiol proteinase inhibitors ubiquitously present in many plants as well as animal tissues. In the present study, a cystatin like a thiol proteinase inhibitor (rCTPI) gene was isolated from chickpea plant and cloned in pET28a vector. The recombinant vector with CTPI gene was expressed and recombinant CTPI proteins was isolated and purified. The purified rCTPI was physiologically active at pH 7.5 and temperature range of 35-37⁰ C. Larvicidal activity of rCTPI were determined with a potential application as bio-pesticide.