In-Vitro Assessment Of Antioxidant Activity, Total Phenolic And Flavonoid Content For Various Extracts Of Gardenia Resinifera Roth

Abstract

Gardenia resinifera (Rubiaceae) stem possess traditional uses such as  Anti-spasmodic, Expectorant, Anti-microbial, Anti-helmintic, Anti-epileptic, Anti-convulsant properties due to presence  of complex phytochemicals. In the present study we evaluated Phenolic, Flavonoid and  Antioxidant activity of Hexane, Ethylacetate, methanol and  Aq.methanolic extracts of stems of Gardinea resinifera  by application of Folin Cio-calteu assay method, Aluminium Chloride assay method and DPPH radical scavenging assay, respectively The results  indicated high amount of phenolic and flavonoid content in Aq.Methanoic extract. The Aq.methanoic extract was found to display highest TPC (46.60 mg GAE/g) and  TFC(158.06mg QE/g, 55.63mg CE/g) , interestingly the better anti-oxidant activity was also found in Aq.methanolic extract with the percentage inhibition rate 44.59% at 50µg/ mL. The results suggested that G.resinifera has the potential to be source of alternative medicine due to its reportedly good Anti-Oxidant activity.