Isolation and Molecular Identification of Salmonella Typhi Using Polymerase Chain Reaction (PCR) Technique

Abstract

Salmonellosis is a disease condition caused by a large group of bacteria of the genus Salmonella that can affect human being throughout the world. Salmonella infection remains as a serious problem to public health and causes substantial economic losses resulting from mortality, morbidity. Food poisoning with gastroenteritis to human represents a serious problem for the food industry. Traditional methods of identification of Salmonella typhi and its related non-typhoidal food borne pathogens organisms are laborious and time consuming. So there is an immediate need for new, innovative, faster and and reliable methods for identification of Salmonella Typhi. Recent developments in the fields of Molecular Biology, have paved the for such methods and have revolutionized the detection of pathogenic microbes at molecular level. Discovery of Polymerase Chain Reaction Technology was a major step in this direction. The present study aimed at characterization of the isolated Salmonella serovars using Morphological, Biochemical, and Molecular Techniques using Polymerase Chain Reaction Technology. The isolated organism was identified as belonging to Salmonella species my Morphological and Biochemical Tests and confirmed by PCR amplification and matching with specific primers for the species. The results of Agarose Gel Electrophoresis revealed a band of 420bp with HisJ and 466bp with Vi LPS.